Cdk2 Antibody Cell Signaling

Mix well then add 0 1 g bsa mix.

Cdk2 antibody cell signaling.

Anti rabbit igg h l f ab 2 fragment alexa fluor 488 conjugate 4412. Lane 1 is 10 input. Inhibitory phosphorylation occurs on thr14 and tyr15 1. Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery.

Western blot analysis was performed using cdk12 antibody. Wild type hap1 cell lysate 20 µg lanes 2 4 and 6. Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery. Phospho cdk2 thr160 antibody detects endogenous levels of cdk2 only when phosphorylated at threonine 160.

An antibody shouldn t be one of the variables in your experiment. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding thr160 of human cdk2. Cdk2 cell division protein kinase 2 cyclin dependent kinase 2 cdc2 related protein kinase cell devision kinase 2 p33 protein kinase p33 cdk2 immunogen. Recommended fluorochrome conjugated anti rabbit secondary antibodies.

Kinase activity associated with cyclin a cdc2 is found only in g2 phase. Inhibitory phosphorylation occurs on thr14 and tyr15 1. Cdk2 antibodies cdk2 antibodies p33cdk2 associates with cyclin a in human cells. Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.

Antibodies are purified by protein a and peptide affinity chromatography. Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor. Red signal from loading control ab8226 observed at 42 kda lanes 5 and 6. Merged red and green signal.

Lanes 1 3 and 5. Find out why customers rank cst highest for antibody specificity and sensitivity. Ab32147 was shown to specifically react with cdk2 when cdk2 knockout samples were used. Green signal from target ab32147 observed at 34 kda lanes 3 and 4.

To prepare 10 ml add 30 µl triton x 100 to 10 ml 1x pbs. Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery. It does not cross react with other phosphorylated cyclin dependent kinases. Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.

Inhibitory phosphorylation occurs on thr14 and tyr15 1. Antibody dilution buffer 1x pbs 1 bsa 0 3 triton x 100.