Cellulose Acetate Paper Electrophoresis

First the cellulose acetate filter paper moistens with the buffer solution.

Cellulose acetate paper electrophoresis.

Cellulose acetate is the acetate ester of cellulose it was first prepared in 1865. On cathode side of midline current 0 4 ma cm width of paper. It involves the following steps. Provides a good background or staining glycoprotein application.

It is also used as a synthetic fiber in the manufacture of cigarette filters and playing cards in photographic film cellulose acetate replaced nitrate film in the 1950s being far. After staining strips are made transparent by soaking in whitmore s oil or liquid paraffin and then suitable for transmittance densitometry of the protein bands. Here we describe a modified version of cellulose acetate membrane electrophoresis for hemoglobin separation from blood sample. The electrophoresis on cellulose acetate membrane is most widely used because of its simplicity and is without the use of any sophisticated instrument other than electrophoresis apparatus and the cellulose acetate strip.

The cellulose acetate system is the ideal tank for both standard and wet cellulose acetate electrophoresis. It gives sharper bands. Electrophoresis is typically performed at ph 8 6 using cellulose acetate as the support medium. Cellulose acetate electrophoresis 23.

Cellulose acetate electrophoresis is more or less very similar to the paper electrophoresis. Cellulose acetate electrophoresis it contains 2 3 acetyl groups per glucose unit and its adsorption capacity is less than that of paper. Alternative to paper electrophoresis. 12x2 5 cm sample applied from micropipette or capillary along a line about 1 cm.

At this ph the overall hemoglobin molecule is negatively charged and when placed in an electric field will move towards the positive terminal anode. Cellulose acetate electrophoresis the helena electrophoresis system helena laboratories beaumont tx usa was used to electrophorese hemolysates on titan iii cellulose acetate plates at 350 v for 25 min in an alkaline buffer. Cellulose acetate is used as a film base in photography as a component in some coatings and as a frame material for eyeglasses. This new range of cellulose acetate products offers a complete system solution for research and clinical cellulose acetate electrophoresis procedures.

The results were analyzed according to manufacturer s guidelines. After this immerse both the ends of cellulose acetate filter paper in a buffer solution. Widely used in analysis of clinical and biological protein sample albumin and globulins. It is a modified version of paper electrophore sis developed by kohn in 1958.